5CHE

Crystal structure of Arabidopsis glutamyl-tRNA reductase in complex with its regulatory proteins

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Glutamyl-tRNA reductase 1, chloroplastic	polymer	472	52139.9	2	UniProt (P42804) Pfam (PF05201) Pfam (PF01488) Pfam (PF00745) In PDB	Arabidopsis thaliana (Mouse-ear cress)	GluTR
2	C, D	Glutamyl-tRNA reductase-binding protein, chloroplastic	polymer	310	34272.6	2	UniProt (Q9LU39) Pfam (PF10615) In PDB	Arabidopsis thaliana (Mouse-ear cress)	GluTR-binding protein,Protein PROTON GRADIENT REGULATION 7
3	E, F	Protein FLUORESCENT IN BLUE LIGHT, chloroplastic	polymer	159	17771.3	2	UniProt (Q940U6) Pfam (PF13424) In PDB	Arabidopsis thaliana (Mouse-ear cress)
4		water	water		18.0	6	Chemie (HOH)

Sequence modifications

A, B: 73 - 543 (UniProt: P42804)

PDB	External Database	Details
Ser 72	-	expression tag

C, D: 42 - 317 (UniProt: Q9LU39)

PDB	External Database	Details
Met 8	-	initiating methionine
Gly 9	-	expression tag
Ser 10	-	expression tag
Ser 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
Ser 18	-	expression tag
Ser 19	-	expression tag
Gly 20	-	expression tag
Leu 21	-	expression tag
Val 22	-	expression tag
Pro 23	-	expression tag
Arg 24	-	expression tag
Gly 25	-	expression tag
Ser 26	-	expression tag
His 27	-	expression tag
Met 28	-	expression tag
Ala 29	-	expression tag
Ser 30	-	expression tag
Met 31	-	expression tag
Thr 32	-	expression tag
Gly 33	-	expression tag
Gly 34	-	expression tag
Gln 35	-	expression tag
Gln 36	-	expression tag
Met 37	-	expression tag
Gly 38	-	expression tag
Arg 39	-	expression tag
Gly 40	-	expression tag
Ser 41	-	expression tag

E, F: 196 - 317 (UniProt: Q940U6)

PDB	External Database	Details
Met 159	-	initiating methionine
Lys 160	-	expression tag
Tyr 161	-	expression tag
Leu 162	-	expression tag
Leu 163	-	expression tag
Pro 164	-	expression tag
Thr 165	-	expression tag
Ala 166	-	expression tag
Ala 167	-	expression tag
Ala 168	-	expression tag
Gly 169	-	expression tag
Leu 170	-	expression tag
Leu 171	-	expression tag
Leu 172	-	expression tag
Leu 173	-	expression tag
Ala 174	-	expression tag
Ala 175	-	expression tag
Gln 176	-	expression tag
Pro 177	-	expression tag
Ala 178	-	expression tag
Met 179	-	expression tag
Ala 180	-	expression tag
Met 181	-	expression tag
Asp 182	-	expression tag
Ile 183	-	expression tag
Gly 184	-	expression tag
Ile 185	-	expression tag
Asn 186	-	expression tag
Ser 187	-	expression tag
Asp 188	-	expression tag
Pro 189	-	expression tag
His 190	-	expression tag
His 191	-	expression tag
His 192	-	expression tag
His 193	-	expression tag
His 194	-	expression tag
His 195	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	208367.6
All*	Total formula weight	208367.6

*Water molecules are not included.