5CE3

The Yersinia YopO - actin complex with MgADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Actin	polymer	376	41755.6	2	UniProt (G3CKA6) Pfam (PF00022) In PDB	Spodoptera frugiperda (Fall armyworm)
2	B, D	Protein kinase YopO	polymer	643	71776.9	2	UniProt (Q93KQ6) Pfam (PF00069) Pfam (PF09632) In PDB	Yersinia enterocolitica	YopO
3	A, C	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
4	A, C	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	B, D	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
6	B, D	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7		water	water		18.0	9	Chemie (HOH)

Sequence modifications

B, D: 89 - 729 (UniProt: Q93KQ6)

PDB	External Database	Details
Gly 87	-	expression tag
Pro 88	-	expression tag
Tyr 205	Lys 205	engineered mutation
Tyr 206	Glu 206	engineered mutation
Tyr 207	Glu 207	engineered mutation
Tyr 440	Lys 440	engineered mutation
Tyr 441	Lys 441	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	227065.0
Non-Polymers*	Number of molecules	8
	Total formula weight	1997.5
All*	Total formula weight	229062.5

*Water molecules are not included.