5C8E

Crystal structure of Thermus thermophilus CarH bound to adenosylcobalamin and a 26-bp DNA segment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Light-dependent transcriptional regulator CarH	polymer	305	33323.3	8	UniProt (Q746J7) Pfam (PF13411) Pfam (PF02607)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
2	I, K (I, K)	26-mer DNA segment containing the CarH operator sequence (antisense strand)	polymer	26	7998.2	2		Thermus thermophilus HB27
3	J, L (J, L)	26-mer DNA segment containing the CarH operator sequence (sense strand)	polymer	26	7972.2	2		Thermus thermophilus HB27
4	AA, M, O, Q, S... (H, A, B, C, D...)	COBALAMIN	non-polymer		1330.4	8	Chemie (B12)
5	BA, N, P, R, T... (H, A, B, C, D...)	5'-DEOXYADENOSINE	non-polymer		251.2	8	Chemie (5AD)

Sequence modifications

A, B, C, D, E, F, G, H: 1 - 285 (UniProt: Q746J7)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	298526.8
Non-Polymers*	Number of molecules	16
	Total formula weight	12652.8
All*	Total formula weight	311179.5

*Water molecules are not included.