5C7J

CRYSTAL STRUCTURE OF NEDD4 WITH A UB VARIANT

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	E3 ubiquitin-protein ligase NEDD4	polymer	382	45427.7	2	UniProt (P46934) Pfam (PF00632)	Homo sapiens (Human)	Cell proliferation-inducing gene 53 protein,Neural precursor cell expressed developmentally down-regulated protein 4,NEDD-4
2	C, D (C, D)	Polyubiquitin-C	polymer	92	10503.9	2	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens (Human)
3	E, F (A, B)	water	water		18.0	4	Chemie (HOH)

Sequence modifications

A, B: 520 - 900 (UniProt: P46934)

PDB	External Database	Details
Gly 519	-	expression tag

C, D: 1 - 74 (UniProt: P0CG48)

PDB	External Database	Details
Ala -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Val -10	-	expression tag
Thr -9	-	expression tag
Ser -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Lys -4	-	expression tag
Ala -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Thr 0	-	expression tag
Ala 9	Thr 9	engineered mutation
Trp 11	Lys 11	engineered mutation
Gly 12	Thr 12	engineered mutation
Arg 62	Gln 62	engineered mutation
Tyr 63	Lys 63	engineered mutation
Asp 64	Glu 64	engineered mutation
Gln 66	Thr 66	engineered mutation
Gly 71	Leu 71	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	111863.2
All*	Total formula weight	111863.2

*Water molecules are not included.