5C6T

Crystal structure of HCMV glycoprotein B in complex with 1G2 Fab

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Envelope glycoprotein B	polymer	636	73034.3	1	UniProt (P13201) Pfam (PF17416) Pfam (PF17417) Pfam (PF00606)	Human cytomegalovirus (HHV-5)	gB
2	B (H)	1G2 Fab heavy chain	polymer	233	25302.3	1		Homo sapiens
3	C (L)	1G2 Fab light chain	polymer	214	22853.1	1		Homo sapiens
4	D (B)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
5	E, F, G, H, I... (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	8	Chemie (NAG)
6	M (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 87 - 698 (UniProt: P13201)

PDB	External Database	Details
Met 63	-	expression tag
Glu 64	-	expression tag
Ser 65	-	expression tag
Arg 66	-	expression tag
Ile 67	-	expression tag
Trp 68	-	expression tag
Cys 69	-	expression tag
Leu 70	-	expression tag
Val 71	-	expression tag
Val 72	-	expression tag
Cys 73	-	expression tag
Val 74	-	expression tag
Asn 75	-	expression tag
Leu 76	-	expression tag
Cys 77	-	expression tag
Ile 78	-	expression tag
Val 79	-	expression tag
Cys 80	-	expression tag
Leu 81	-	expression tag
Gly 82	-	expression tag
Ala 83	-	expression tag
Ala 84	-	expression tag
Val 85	-	expression tag
Ser 86	-	expression tag
His 156	Ile 156	engineered mutation
Arg 157	His 157	engineered mutation
Asn 240	Trp 240	engineered mutation
Thr 242	Tyr 242	engineered mutation
Ser 246	Cys 246	engineered mutation
Ser 457	Arg 457	engineered mutation
Ser 460	Arg 460	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	121189.7
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	9
	Total formula weight	1794.0
All*	Total formula weight	123408.1

*Water molecules are not included.