5C2F

K428A mutant nuclease domain of the large terminase subunit gp2 of bacterial virus Sf6 with Manganese and beta-thujaplicinol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Gene 2 protein	polymer	278	30721.6	1	UniProt (Q716H3) In PDB	Enterobacteria phage Sf6 (Shigella flexneri bacteriophage VI)
2	A	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	A	2,7-dihydroxy-4-(propan-2-yl)cyclohepta-2,4,6-trien-1-one	non-polymer		180.2	1	Chemie (JTH)
4		water	water		18.0	128	Chemie (HOH)

Sequence modifications

A: 213 - 470 (UniProt: Q716H3)

PDB	External Database	Details
Met 193	-	expression tag
Gly 194	-	expression tag
Ser 195	-	expression tag
Ser 196	-	expression tag
His 197	-	expression tag
His 198	-	expression tag
His 199	-	expression tag
His 200	-	expression tag
His 201	-	expression tag
His 202	-	expression tag
Ser 203	-	expression tag
Ser 204	-	expression tag
Gly 205	-	expression tag
Leu 206	-	expression tag
Val 207	-	expression tag
Pro 208	-	expression tag
Arg 209	-	expression tag
Gly 210	-	expression tag
Ser 211	-	expression tag
His 212	-	expression tag
Ala 428	Lys 428	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	30721.6
Non-Polymers*	Number of molecules	3
	Total formula weight	290.1
All*	Total formula weight	31011.7

*Water molecules are not included.