5BYW
Crystal structure of engineered trifunctional CtCEL5E
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E | Endoglucanase H | polymer | 414 | 47372.7 | 5 | UniProt (P16218) Pfam (PF00150) In PDB | Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) | Cellulase H,Endo-1,4-beta-glucanase H,EgH |
| 2 | water | water | 18.0 | 176 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E: 39 - 414 (UniProt: P16218)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Gly 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Ser 4 | - | expression tag |
| His 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| His 8 | - | expression tag |
| His 9 | - | expression tag |
| His 10 | - | expression tag |
| Ser 11 | - | expression tag |
| Ser 12 | - | expression tag |
| Gly 13 | - | expression tag |
| Leu 14 | - | expression tag |
| Val 15 | - | expression tag |
| Pro 16 | - | expression tag |
| Arg 17 | - | expression tag |
| Gly 18 | - | expression tag |
| Ser 19 | - | expression tag |
| His 20 | - | expression tag |
| Met 21 | - | expression tag |
| Ala 22 | - | expression tag |
| Ser 23 | - | expression tag |
| Met 24 | - | expression tag |
| Thr 25 | - | expression tag |
| Gly 26 | - | expression tag |
| Gly 27 | - | expression tag |
| Gln 28 | - | expression tag |
| Gln 29 | - | expression tag |
| Met 30 | - | expression tag |
| Gly 31 | - | expression tag |
| Arg 32 | - | expression tag |
| Ile 33 | - | expression tag |
| Glu 34 | - | expression tag |
| Gly 35 | - | expression tag |
| Arg 36 | - | expression tag |
| Glu 37 | - | expression tag |
| Phe 38 | - | expression tag |
| Ala 267 | Phe 518 | engineered mutation |
| Phe 273 | Tyr 524 | engineered mutation |
| Gln 278 | - | insertion |
| Gly 279 | - | insertion |
| Ala 280 | - | insertion |
| Glu 281 | - | insertion |
| Trp 282 | - | insertion |
| Val 283 | - | insertion |
| Glu 284 | - | insertion |
| Gly 285 | - | insertion |
| Ser 286 | - | insertion |
| Glu 287 | - | insertion |
| Lys 288 | - | insertion |
| Trp 289 | Lys 529 | engineered mutation |
| Leu 290 | Trp 530 | engineered mutation |
| Gly 291 | Arg 531 | engineered mutation |
| Arg 292 | Gly 532 | engineered mutation |
| Lys 293 | Thr 533 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 236863.3 | |
| All* | Total formula weight | 236863.3 |
