5BWL

Crystal Structure of SIRT5 in Complex with a Coumarin-Labelled Succinyl Peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	NAD-dependent protein deacylase sirtuin-5, mitochondrial	polymer	290	31584.9	1	UniProt (Q9NXA8) Pfam (PF02146)	Homo sapiens (Human)	Regulatory protein SIR2 homolog 5,SIR2-like protein 5
2	B (B)	Coumarin-labelled succinyl peptide	polymer	3	416.5	1		synthetic construct
3	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	D (B)	7-AMINO-4-METHYL-CHROMEN-2-ONE	non-polymer		175.2	1	Chemie (MCM)
5	E, F (A, B)	water	water		18.0	376	Chemie (HOH)

Sequence modifications

A: 33 - 302 (UniProt: Q9NXA8)

PDB	External Database	Details
Met 13	-	expression tag
Gly 14	-	expression tag
Ser 15	-	expression tag
Ser 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
Ser 23	-	expression tag
Ser 24	-	expression tag
Gly 25	-	expression tag
Leu 26	-	expression tag
Val 27	-	expression tag
Pro 28	-	expression tag
Arg 29	-	expression tag
Gly 30	-	expression tag
Ser 31	-	expression tag
His 32	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	32001.4
Non-Polymers*	Number of molecules	2
	Total formula weight	240.6
All*	Total formula weight	32242.0

*Water molecules are not included.