5BWE

Benzylsuccinate synthase alpha-beta-gamma complex with bound toluene and fumarate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	benzylsuccinate synthase alpha chain	polymer	878	99117.1	2	UniProt (O68395) Pfam (PF02901) Pfam (PF01228)	Thauera aromatica	TutD
2	B, E (B, F)	benzylsuccinate synthase beta chain	polymer	81	9303.3	2	UniProt (O68396) Pfam (PF18512)	Thauera aromatica	TutG
3	C, F (C, G)	benzylsuccinate synthase gamma chain	polymer	60	6865.7	2	UniProt (O68394) Pfam (PF08201)	Thauera aromatica	TutF
4	G, J (A, D)	FUMARIC ACID	non-polymer		116.1	2	Chemie (FUM)
5	H, K (A, D)	TOLUENE	non-polymer		92.1	2	Chemie (MBN)
6	I, L (B, F)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)

Sequence modifications

A, D: 2 - 865 (UniProt: O68395)

PDB	External Database	Details
Ile 789	Met 788	engineered mutation
Ser 866	-	expression tag
Gly 867	-	expression tag
Thr 868	-	expression tag
Gly 869	-	expression tag
Ser 870	-	expression tag
Gly 871	-	expression tag
Ser 872	-	expression tag
Ser 873	-	expression tag
His 874	-	expression tag
His 875	-	expression tag
His 876	-	expression tag
His 877	-	expression tag
His 878	-	expression tag
His 879	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	230572.2
Non-Polymers*	Number of molecules	6
	Total formula weight	1119.7
All*	Total formula weight	231691.9

*Water molecules are not included.