5BRP
Crystal structure of Bacillus licheniformis trehalose-6-phosphate hydrolase (TreA), mutant R201Q, in complex with PNG
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Glycoside Hydrolase Family 13 | polymer | 568 | 66613.2 | 4 | UniProt (Q65MI2) Pfam (PF00128) Pfam (PF16657) In PDB | Bacillus licheniformis ATCC 14580 = DSM 13 | trehalose-6-phosphate hydrolase |
2 | A, B, C | 4-nitrophenyl alpha-D-glucopyranoside | non-polymer | 301.2 | 3 | Chemie (PNG) | |||
3 | A, B, C, D | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
4 | water | water | 18.0 | 1663 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 562 (UniProt: Q65MI2)
PDB | External Database | Details |
---|---|---|
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
His 0 | - | expression tag |
Gln 201 | Arg 201 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 266453.0 | |
Non-Polymers* | Number of molecules | 7 |
Total formula weight | 1001.0 | |
All* | Total formula weight | 267453.9 |