5BJW

X-ray structure of the PglF 4,6-dehydratase from campylobacter jejuni, T595S variant, in complex with UDP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	WlaL protein	polymer	366	40835.4	2	UniProt (O86159) Pfam (PF02719)	Campylobacter jejuni
2	C, I (A, B)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	2	Chemie (UDP)
3	D, H (A, B)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
4	E, J, K, L (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
5	F, G, M, N (A, B)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
6	O, P (A, B)	water	water		18.0	697	Chemie (HOH)

Sequence modifications

A, B: 244 - 590 (UniProt: O86159)

PDB	External Database	Details
Ser 395	Thr 395	engineered mutation
Ala 591	-	expression tag
Ala 592	-	expression tag
Gly 593	-	expression tag
Phe 594	-	expression tag
Asn 595	-	expression tag
Arg 596	-	expression tag
Ile 597	-	expression tag
Pro 598	-	expression tag
Ala 599	-	expression tag
Ala 600	-	expression tag
Ala 601	-	expression tag
Leu 602	-	expression tag
Glu 603	-	expression tag
His 604	-	expression tag
His 605	-	expression tag
His 606	-	expression tag
His 607	-	expression tag
His 608	-	expression tag
His 609	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	81670.8
Non-Polymers*	Number of molecules	12
	Total formula weight	2475.4
All*	Total formula weight	84146.2

*Water molecules are not included.