5BJV

X-ray structure of the PglF UDP-N-acetylglucosamine 4,6-dehydratase from Campylobacterjejuni, D396N/K397A variant in complex with UDP-N-acrtylglucosamine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	WlaL protein	polymer	366	40790.3	2	UniProt (O86159) Pfam (PF02719)	Campylobacter jejuni
2	C, I (A, B)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
3	D, J (A, B)	URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE	non-polymer		607.4	2	Chemie (UD1)
4	E, F, K (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
5	G, H, L, M (A, B)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
6	N, O (A, B)	water	water		18.0	612	Chemie (HOH)

Sequence modifications

A, B: 244 - 590 (UniProt: O86159)

PDB	External Database	Details
Asn 396	Asp 396	engineered mutation
Ala 397	Lys 397	engineered mutation
Ala 591	-	expression tag
Ala 592	-	expression tag
Gly 593	-	expression tag
Phe 594	-	expression tag
Asn 595	-	expression tag
Arg 596	-	expression tag
Ile 597	-	expression tag
Pro 598	-	expression tag
Ala 599	-	expression tag
Ala 600	-	expression tag
Ala 601	-	expression tag
Leu 602	-	expression tag
Glu 603	-	expression tag
His 604	-	expression tag
His 605	-	expression tag
His 606	-	expression tag
His 607	-	expression tag
His 608	-	expression tag
His 609	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	81580.7
Non-Polymers*	Number of molecules	11
	Total formula weight	2819.7
All*	Total formula weight	84400.4

*Water molecules are not included.