5B89

Crystal structure of a Cysteine Desulfurase from Thermococcus onnurineus NA1 in complex with alanine at 1.5 Angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cysteine desulfurase	polymer	419	46704.2	2	UniProt (B6YT87) Pfam (PF00266)	Thermococcus onnurineus (strain NA1)
2	C, H (A, B)	ALANINE	non-polymer		89.1	2	Chemie (ALA)
3	D, G (A, B)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
4	E (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	F (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
6	I, J (A, B)	water	water		18.0	545	Chemie (HOH)

Sequence modifications

A, B: 1 - 399 (UniProt: B6YT87)

PDB	External Database	Details
Met -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	93408.5
Non-Polymers*	Number of molecules	6
	Total formula weight	826.6
All*	Total formula weight	94235.1

*Water molecules are not included.