5B58

Inward-facing conformation of ABC heme importer BhuUV in complex with periplasmic heme binding protein BhuT from Burkholderia cenocepacia

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Putative hemin ABC transport system, membrane protein	polymer	385	38801.5	2	UniProt (B4EKB4) Pfam (PF01032)	Burkholderia cenocepacia J2315	heme importer transmembrane subunit BhuU
2	C, D (C, D)	Hemin import ATP-binding protein HmuV	polymer	273	29232.5	2	UniProt (B4EKB5) Pfam (PF00005)	Burkholderia cenocepacia J2315	heme importer ATP-binding subunit BhuV
3	E (T)	Putative hemin transport system, substrate-binding protein	polymer	271	28016.0	1	UniProt (B4EKB3) Pfam (PF01497)	Burkholderia cenocepacia J2315	periplasmic heme-binding protein BhuT

Sequence modifications

A, B: 1 - 362 (UniProt: B4EKB4)

PDB	External Database	Details
Met -22	-	expression tag
Gly -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
Ile -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
His 0	-	expression tag

T: 40 - 305 (UniProt: B4EKB3)

PDB	External Database	Details
Gly 35	-	expression tag
Pro 36	-	expression tag
Leu 37	-	expression tag
Gly 38	-	expression tag
Ser 39	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	164084.0
All*	Total formula weight	164084.0

*Water molecules are not included.