5B57

Inward-facing conformation of ABC heme importer BhuUV from Burkholderia cenocepacia

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Putative hemin ABC transport system, membrane protein	polymer	385	38801.5	2	UniProt (B4EKB4) Pfam (PF01032)	Burkholderia cenocepacia J2315	heme importer transmembrane subunit BhuU
2	C, D (C, D)	Hemin import ATP-binding protein HmuV	polymer	273	29232.5	2	UniProt (B4EKB5) Pfam (PF00005)	Burkholderia cenocepacia J2315	heme importer ATP-binding subunit BhuV
3	E, F (A, B)	DECYL-BETA-D-MALTOPYRANOSIDE	non-polymer		482.6	2	Chemie (DMU)
4	G, H (C, D)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
5	I, J, K, L (A, B, C, D)	water	water		18.0	22	Chemie (HOH)

Sequence modifications

A, B: 1 - 362 (UniProt: B4EKB4)

PDB	External Database	Details
Met -22	-	expression tag
Gly -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
Ile -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	136068.0
Non-Polymers*	Number of molecules	4
	Total formula weight	1157.3
All*	Total formula weight	137225.2

*Water molecules are not included.