5B4C

Crystal structure of H10N mutant of LpxH with manganese

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	UDP-2,3-diacylglucosamine hydrolase	polymer	248	28646.9	2	UniProt (Q9I2V0) Pfam (PF00149) In PDB	Pseudomonas aeruginosa PAO1	UDP-2,3-diacylglucosamine pyrophosphatase LpxH, UDP-2,3-diacylglucosamine diphosphatase
2	A, B	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	A, B	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4		water	water		18.0	442	Chemie (HOH)

Sequence modifications

A, B: 1 - 240 (UniProt: Q9I2V0)

PDB	External Database	Details
Asn 10	His 10	engineered mutation
Leu 241	-	expression tag
Glu 242	-	expression tag
His 243	-	expression tag
His 244	-	expression tag
His 245	-	expression tag
His 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57293.8
Non-Polymers*	Number of molecules	6
	Total formula weight	478.3
All*	Total formula weight	57772.0

*Water molecules are not included.