5B32

The crystal structure of the heterotypic H2AZ/H2A nucleosome with H3.3.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3.3	polymer	139	15643.3	2	UniProt (P84243) Pfam (PF00125) In PDB	Homo sapiens (Human)
2	B, F	Histone H4	polymer	106	11676.7	2	UniProt (P62805) Pfam (PF15511) In PDB	Homo sapiens (Human)
3	C	Histone H2A type 1-B/E	polymer	133	14447.8	1	UniProt (P04908) Pfam (PF00125) Pfam (PF16211) In PDB	Homo sapiens (Human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H	Histone H2B type 1-J	polymer	129	14217.5	2	UniProt (P06899) Pfam (PF00125) In PDB	Homo sapiens (Human)	Histone H2B.1,Histone H2B.r,H2B/r
5	G	Histone H2A.Z	polymer	131	13864.1	1	UniProt (P0C0S5) Pfam (PF00125) Pfam (PF16211) In PDB	Homo sapiens (Human)	H2A/z
6	I, J	DNA (146-MER)	polymer	146	45053.9	2		Homo sapiens
7	A, C, E, G	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
8	D, E, I, J	MANGANESE (II) ION	non-polymer		54.9	10	Chemie (MN)
9		water	water		18.0	88	Chemie (HOH)

Sequence modifications

A, E: 0 - 135 (UniProt: P84243)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

B, F: 0 - 102 (UniProt: P62805)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

C: 0 - 129 (UniProt: P04908)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

D, H: 0 - 125 (UniProt: P06899)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

G: 0 - 127 (UniProt: P0C0S5)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	201494.6
Non-Polymers*	Number of molecules	14
	Total formula weight	691.2
All*	Total formula weight	202185.8

*Water molecules are not included.