5B12
Crystal structure of the B-type halohydrin hydrogen-halide-lyase mutant F71W/Q125T/D199H from Corynebacterium sp. N-1074
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Halohydrin epoxidase B | polymer | 227 | 25358.5 | 6 | UniProt (Q46347) Pfam (PF13561) | Corynebacterium sp. | |
| 2 | G, H, I, J, K... (A, B, C, D, E...) | CHLORIDE ION | non-polymer | 35.5 | 6 | Chemie (CL) | |||
| 3 | M, N, O, P, Q... (A, B, C, D, E...) | water | water | 18.0 | 1524 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F: 3 - 227 (UniProt: Q46347)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Lys 2 | - | expression tag |
| Trp 71 | Phe 79 | engineered mutation |
| Thr 125 | Gln 133 | engineered mutation |
| His 199 | Asp 207 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 152151.0 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 212.7 | |
| All* | Total formula weight | 152363.7 |
