5AZA

Crystal structure of MBP-sAglB fusion protein with a 20-residue spacer in the connector helix

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Maltose-binding periplasmic protein,Oligosaccharyl transferase stt3 subunit related protein	polymer	872	97120.0	1	UniProt (P0AEX9) UniProt (I6V0B8) Pfam (PF01547)	Escherichia coli	MBP,MMBP,Maltodextrin-binding protein
2	B (B)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	C (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4	D (A)	water	water		18.0	126	Chemie (HOH)

Sequence modifications

A: 1 - 368 (UniProt: P0AEX9)

PDB	External Database	Details
Met 0	-	initiating methionine
Val 312	Ala 338	engineered mutation
Ala 369	-	linker
Ala 370	-	linker
Ala 371	-	linker
Lys 372	-	linker
Ala 373	-	linker
Glu 374	-	linker
Ala 375	-	linker
Ala 376	-	linker
Ala 377	-	linker
Lys 378	-	linker
Ala 379	-	linker
Glu 380	-	linker
Ala 381	-	linker
Ala 382	-	linker
Ala 383	-	linker
Lys 384	-	linker
Ala 385	-	linker
Ala 386	-	linker
Ala 387	-	linker
Ala 388	-	linker

A: 389 - 865 (UniProt: I6V0B8)

PDB	External Database	Details
His 866	-	expression tag
His 867	-	expression tag
His 868	-	expression tag
His 869	-	expression tag
His 870	-	expression tag
His 871	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	97120.0
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	1
	Total formula weight	40.1
All*	Total formula weight	97502.3

*Water molecules are not included.