5AZA
Crystal structure of MBP-sAglB fusion protein with a 20-residue spacer in the connector helix
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Maltose-binding periplasmic protein,Oligosaccharyl transferase stt3 subunit related protein | polymer | 872 | 97120.0 | 1 | UniProt (P0AEX9) UniProt (I6V0B8) Pfam (PF01547) Pfam (PF18246) Pfam (PF21618) Pfam (PF18235) In PDB | Escherichia coli | MBP,MMBP,Maltodextrin-binding protein |
2 | B | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
3 | A | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
4 | water | water | 18.0 | 126 | Chemie (HOH) |
Sequence modifications
A: 1 - 368 (UniProt: P0AEX9)
A: 389 - 865 (UniProt: I6V0B8)
PDB | External Database | Details |
---|---|---|
Met 0 | - | initiating methionine |
Val 312 | Ala 338 | engineered mutation |
Ala 369 | - | linker |
Ala 370 | - | linker |
Ala 371 | - | linker |
Lys 372 | - | linker |
Ala 373 | - | linker |
Glu 374 | - | linker |
Ala 375 | - | linker |
Ala 376 | - | linker |
Ala 377 | - | linker |
Lys 378 | - | linker |
Ala 379 | - | linker |
Glu 380 | - | linker |
Ala 381 | - | linker |
Ala 382 | - | linker |
Ala 383 | - | linker |
Lys 384 | - | linker |
Ala 385 | - | linker |
Ala 386 | - | linker |
Ala 387 | - | linker |
Ala 388 | - | linker |
PDB | External Database | Details |
---|---|---|
His 866 | - | expression tag |
His 867 | - | expression tag |
His 868 | - | expression tag |
His 869 | - | expression tag |
His 870 | - | expression tag |
His 871 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 97120.0 | |
Branched | Number of molecules | 1 |
Total formula weight | 342.3 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 40.1 | |
All* | Total formula weight | 97502.3 |