5AZ6
Crystal structure of MBP-Tom20 fusion protein with a 2-residue spacer in the connector helix
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (B, A) | Maltose-binding periplasmic protein,Mitochondrial import receptor subunit TOM20 homolog | polymer | 433 | 47860.4 | 2 | UniProt (P0AEX9) UniProt (Q62760) Pfam (PF01547) Pfam (PF02064) | Escherichia coli (strain K12) | MBP,MMBP,Maltodextrin-binding protein,Mitochondrial 20 kDa outer membrane protein,Outer mitochondrial membrane receptor Tom20 |
| 2 | C, D (C, D) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 3 | E, F (B, A) | water | water | 18.0 | 21 | Chemie (HOH) |
Sequence modifications
B, A: 2 - 369 (UniProt: P0AEX9)
B, A: 372 - 433 (UniProt: Q62760)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Val 313 | Ala 338 | engineered mutation |
| Lys 370 | - | linker |
| Glu 371 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 95720.8 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 684.6 | |
| All* | Total formula weight | 96405.4 |
