5AXH

Crystal structure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus, D312G mutant in complex with isomaltohexaose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Dextranase	polymer	618	71327.5	2	UniProt (B0KBZ7) Pfam (PF13199) In PDB	Thermoanaerobacter pseudethanolicus ATCC 33223
2	C	alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose	branched		666.6	1	In PDB GlyTouCan (G80662SX)
3	D	alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-beta-D-glucopyranose	branched		990.9	1	In PDB GlyTouCan (G26640RK)
4	A, B	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4)
5	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6		water	water		18.0	594	Chemie (HOH)

Sequence modifications

A, B: 3 - 610 (UniProt: B0KBZ7)

PDB	External Database	Details
Met 1	-	expression tag
Gly 2	-	expression tag
Gly 312	Asp 312	engineered mutation
Leu 611	-	expression tag
Glu 612	-	expression tag
His 613	-	expression tag
His 614	-	expression tag
His 615	-	expression tag
His 616	-	expression tag
His 617	-	expression tag
His 618	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	142655.0
Branched	Number of molecules	2
	Total formula weight	1657.4
Non-Polymers*	Number of molecules	6
	Total formula weight	564.1
All*	Total formula weight	144876.5

*Water molecules are not included.