5ARD
Cooperative bio-metallic selectivity in a tailored protease enables creation of a C-C cross-coupling Heckase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | SUBTILISIN SAVINASE | polymer | 269 | 26708.4 | 1 | UniProt (P29600) Pfam (PF00082) | BACILLUS LENTUS | ALKALINE PROTEASE, SUBTILISIN |
| 2 | B (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 3 | C, D (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 4 | E (A) | 5-methyl-2-(5-methylpyridin-2-yl)pyridine | non-polymer | 184.2 | 1 | Chemie (EI3) | |||
| 5 | F (A) | NICKEL (II) ION | non-polymer | 58.7 | 1 | Chemie (NI) | |||
| 6 | G (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 7 | H (A) | water | water | 18.0 | 226 | Chemie (HOH) |
Sequence modifications
A: 1 - 275 (UniProt: P29600)
| PDB | External Database | Details |
|---|---|---|
| Cys 217 | Leu 211 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 26708.4 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 563.3 | |
| All* | Total formula weight | 27271.6 |
