5AQL
Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | HEAT SHOCK COGNATE 71 KDA PROTEIN | polymer | 386 | 42506.1 | 2 | UniProt (P11142) Pfam (PF00012) | HOMO SAPIENS (HUMAN) | HEAT SHOCK 70 KDA PROTEIN 8, LIPOPOLYSACCHARIDE-ASSOCIATED PROTEIN 1, LAP-1, LPS-ASSOCIATED PROTEIN 1 |
| 2 | B, D (B, D) | BAG FAMILY MOLECULAR CHAPERONE REGULATOR 1 | polymer | 118 | 13511.6 | 2 | UniProt (Q99933) Pfam (PF02179) | HOMO SAPIENS (HUMAN) | BAG-1, BCL-2-ASSOCIATED ATHANOGENE 1 |
| 3 | E, F, J, K (A, C) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 4 | Chemie (TRS) | |||
| 4 | G, H, I, L, M (B, C, D) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 5 | N, O, P, Q (A, B, C, D) | water | water | 18.0 | 908 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 381 (UniProt: P11142)
B, D: 151 - 263 (UniProt: Q99933)
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Leu -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Trp 275 | Ser 275 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 146 | - | expression tag |
| Pro 147 | - | expression tag |
| Leu 148 | - | expression tag |
| Gly 149 | - | expression tag |
| Ser 150 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 112035.4 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 949.0 | |
| All* | Total formula weight | 112984.4 |
