5AQ9

DARPin-based Crystallization Chaperones exploit Molecular Geometry as a Screening Dimension in Protein Crystallography

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	OFF7_DB08V4	polymer	422	45678.4	2		SYNTHETIC CONSTRUCT
2	B, D (B, D)	MALTOSE-BINDING PERIPLASMIC PROTEIN	polymer	395	43172.6	2	UniProt (P0AEX9) Pfam (PF01547)	ESCHERICHIA COLI	MBP, MMBP, MALTODEXTRIN-BINDING PROTEIN
3	E, F, G, H (A, B, C, D)	water	water		18.0	1059	Chemie (HOH)

Sequence modifications

B, D: 22 - 381 (UniProt: P0AEX9)

PDB	External Database	Details
Met 1	-	initiating methionine
Arg 2	-	expression tag
Gly 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Gly 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Ser 14	-	expression tag
Met 15	-	expression tag
Lys 16	-	expression tag
Thr 17	-	expression tag
Glu 18	-	expression tag
Glu 19	-	expression tag
Gly 20	-	expression tag
Asn 21	-	expression tag
Gly 382	-	expression tag
Ser 383	-	expression tag
Gly 384	-	expression tag
Gly 385	-	expression tag
Thr 386	-	expression tag
Pro 387	-	expression tag
Gly 388	-	expression tag
Arg 389	-	expression tag
Pro 390	-	expression tag
Ala 391	-	expression tag
Ala 392	-	expression tag
Lys 393	-	expression tag
Leu 394	-	expression tag
Asn 395	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	177702.2
All*	Total formula weight	177702.2

*Water molecules are not included.