5APW

Sequence MATKDD inserted between GCN4 adaptors - Structure T6

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	GENERAL CONTROL PROTEIN GCN4	polymer	92	11069.7	3	UniProt (P03069) Pfam (PF07716)	SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)	AMINO ACID BIOSYNTHESIS REGULATORY PROTEIN, ARTIFICIAL CONSTRUCT
2	D (A)	UREA	non-polymer		60.1	1	Chemie (URE)
3	E (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	F (B)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
5	G, H, I (A, B, C)	water	water		18.0	190	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 28 (UniProt: P03069)

PDB	External Database	Details
Met -25	-	expression tag
Lys -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Pro -17	-	expression tag
Met -16	-	expression tag
Ser -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Val 15	Asn 264	engineered mutation

A, B, C: 35 - 66 (UniProt: P03069)

PDB	External Database	Details
Met 29	-	linker
Ala 30	-	linker
Thr 31	-	linker
Lys 32	-	linker
Asp 33	-	linker
Asp 34	-	linker
Val 49	Asn 264	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	33209.2
Non-Polymers*	Number of molecules	3
	Total formula weight	135.6
All*	Total formula weight	33344.8

*Water molecules are not included.