5APP

Actinobacillus actinomycetemcomitans OMP100 residues 133-198 fused to GCN4 adaptors

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	General control protein GCN4,GENERAL CONTROL PROTEIN GCN4, OUTER MEMBRANE PROTEIN 100,General control protein GCN4	polymer	133	15441.7	3	UniProt (P03069) Pfam (PF07716)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Amino acid biosynthesis regulatory protein,Amino acid biosynthesis regulatory protein
2	D, E, F, G, H (A, B, C)	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
3	I, J, K (A, B, C)	water	water		18.0	35	Chemie (HOH)

Sequence modifications

A, B, C: 104 - 131 (UniProt: P03069)

PDB	External Database	Details
Ile 107	Leu 253	engineered mutation
Ile 111	Val 257	engineered mutation
Ile 114	Leu 260	engineered mutation
Ile 118	Asn 264	engineered mutation
Ile 121	Leu 267	engineered mutation
Ile 125	Val 271	engineered mutation
Ile 128	Leu 274	engineered mutation

A, B, C: 200 - 227 (UniProt: P03069)

PDB	External Database	Details
Ile 203	Leu 253	engineered mutation
Ile 207	Val 257	engineered mutation
Ile 210	Leu 260	engineered mutation
Ile 214	Asn 264	engineered mutation
Ile 217	Leu 267	engineered mutation
Ile 221	Val 271	engineered mutation
Ile 224	Leu 274	engineered mutation
Ile 228	-	expression tag
Lys 229	-	expression tag
Leu 230	-	expression tag
His 231	-	expression tag
His 232	-	expression tag
His 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag
His 236	-	expression tag

A, B, C: 132 - 199 (PDB: 5APP)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	46325.2
Non-Polymers*	Number of molecules	5
	Total formula weight	177.3
All*	Total formula weight	46502.4

*Water molecules are not included.