5AKD

MutS in complex with the N-terminal domain of MutL - crystal form 3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F, I... (A, B, E, F, I...)	DNA MISMATCH REPAIR PROTEIN MUTS	polymer	800	89476.1	6	UniProt (P23909) Pfam (PF01624) Pfam (PF05188) Pfam (PF05192) Pfam (PF05190) Pfam (PF00488)	ESCHERICHIA COLI
2	C, D, G, H, K... (C, D, G, H, K...)	DNA MISMATCH REPAIR PROTEIN MUTL	polymer	369	41275.7	6	UniProt (P23367) Pfam (PF13589) Pfam (PF01119)	ESCHERICHIA COLI
3	M, N, O, P, Q... (A, B, E, F, I...)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	6	Chemie (ANP)

Sequence modifications

A, B, E, F, I, J: 1 - 800 (UniProt: P23909)

PDB	External Database	Details
Ala 93	Cys 93	engineered mutation
Ser 235	Cys 235	engineered mutation
Ala 239	Cys 239	engineered mutation
Cys 246	Asp 246	engineered mutation
Ser 297	Cys 297	engineered mutation
Ser 569	Cys 569	engineered mutation
Val 711	Cys 711	engineered mutation

C, D, G, H, K, L: 1 - 349 (UniProt: P23367)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 61	Cys 61	engineered mutation
Cys 131	Asn 131	engineered mutation
Leu 216	Cys 216	engineered mutation
Phe 256	Cys 256	engineered mutation
Tyr 276	Cys 276	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	784510.9
Non-Polymers*	Number of molecules	6
	Total formula weight	3037.2
All*	Total formula weight	787548.1

*Water molecules are not included.