5AK9

THE CRYSTAL STRUCTURE OF I-DMOI Q42AK120M IN COMPLEX WITH ITS TARGET DNA IN THE PRESENCE OF 2MM MN

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I (A, E, J)	HOMING ENDONUCLEASE I-DMOI	polymer	199	23210.0	3	UniProt (P21505)	DESULFUROCOCCUS MOBILIS
2	B, F, J (B, F, K)	5'-D(*GP*CP*CP*TP*TP*GP*CP*CP*GP*GP*GP*TP*AP*AP)-3'	polymer	14	4296.8	3		SYNTHETIC CONSTRUCT
3	C, G, K (C, G, L)	5'-D(*GP*TP*TP*CP*CP*GP*GP*CP*GP*CP*GP)-3	polymer	11	3366.2	3		SYNTHETIC CONSTRUCT
4	D, H, L (D, H, N)	25MER	polymer	25	7654.9	3		SYNTHETIC CONSTRUCT
5	M, N, O, P, R... (A, E, J)	MANGANESE (II) ION	non-polymer		54.9	6	Chemie (MN)
6	Q (G)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
7	AA, BA, CA, DA, EA... (H, J, K, L, N...)	water	water		18.0	233	Chemie (HOH)

Sequence modifications

A, E, J: 2 - 188 (UniProt: P21505)

PDB	External Database	Details
Ala 1	-	expression tag
Ala 42	Gln 42	conflict
Met 120	Lys 120	conflict
Ala 189	-	expression tag
Ala 190	-	expression tag
Ala 191	-	expression tag
Leu 192	-	expression tag
Glu 193	-	expression tag
His 194	-	expression tag
His 195	-	expression tag
His 196	-	expression tag
His 197	-	expression tag
His 198	-	expression tag
His 199	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	115583.8
Non-Polymers*	Number of molecules	7
	Total formula weight	388.7
All*	Total formula weight	115972.4

*Water molecules are not included.