5AIT

A complex of of RNF4-RING domain, UbeV2, Ubc13-Ub (isopeptide crosslink)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	E3 UBIQUITIN-PROTEIN LIGASE RNF4	polymer	133	14758.2	1	UniProt (O88846) Pfam (PF13639) In PDB	RATTUS NORVEGICUS (NORWAY RAT)	RING FINGER PROTEIN 4, SMALL NUCLEAR RING FINGER PROTEIN, P ROTEIN SNURF, RING DOMAIN
2	B, E	UBIQUITIN-CONJUGATING ENZYME E2 N	polymer	154	17253.8	2	UniProt (P61088) Pfam (PF00179) In PDB	HOMO SAPIENS (HUMAN)	BENDLESS-LIKE UBIQUITIN-CONJUGATING ENZYME, UBC13, UBCH13, UBIQUITIN CARRIER PROTEIN N, UBIQUITIN-PROTEIN LIGASE N
3	C, F	POLYUBIQUITIN-C	polymer	76	8576.8	2	UniProt (P0CH28) Pfam (PF00240) In PDB	BOS TAURUS (CATTLE)
4	D, G	UBIQUITIN-CONJUGATING ENZYME E2 VARIANT 2	polymer	147	16508.9	2	UniProt (Q15819) Pfam (PF00179) In PDB	HOMO SAPIENS (HUMAN)	DDVIT 1, ENTEROCYTE DIFFERENTIATION-ASSOCIATED FACTOR 1, ED AF-1, ENTEROCYTE DIFFERENTIATION-PROMOTING FACTOR 1, EDPF-1, MMS2 HOMOLOG, VITAMIN D3-INDUCIBLE PROTEIN
5	A	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

A: 131 - 194 (UniProt: O88846)

PDB	External Database	Details
Gly 127	-	expression tag
Ala 128	-	expression tag
Met 129	-	expression tag
Gly 130	-	expression tag

A: 196 - 259 (UniProt: O88846)

PDB	External Database	Details
Gly 195	-	linker

B, E: 1 - 152 (UniProt: P61088)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
Lys 87	Cys 87	engineered mutation
Ala 92	Lys 92	engineered mutation

D, G: 1 - 145 (UniProt: Q15819)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	99437.2
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	99698.8

*Water molecules are not included.