5AHF

Crystal structure of Salmonella enterica HisA D7N with ProFAR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase	polymer	253	27130.9	1	UniProt (A0A5T2G6F4) Pfam (PF00977) UniProt (by SIFTS) (P10372)	Salmonella enterica	Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase
2	B (A)	[(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate	non-polymer		577.3	1	Chemie (GUO)
3	C, D (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	E (A)	water	water		18.0	52	Chemie (HOH)

Sequence modifications

A: 1 - 245 (UniProt: A0A5T2G6F4)

PDB	External Database	Details
Asn 7	Asp 7	engineered mutation
Pro 162	Ser 162	engineered mutation
Lys 246	-	expression tag
Gly 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27130.9
Non-Polymers*	Number of molecules	3
	Total formula weight	761.5
All*	Total formula weight	27892.5

*Water molecules are not included.