5AC4

GH20C, Beta-hexosaminidase from Streptococcus pneumoniae in complex with GalNAc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	N-ACETYL-BETA-D-GLUCOSAMINIDASE	polymer	649	74180.5	2	UniProt (C1CH75) Pfam (PF18229) Pfam (PF00728) Pfam (PF18088) UniProt (by SIFTS) (A0A0H2US73) In PDB	STREPTOCOCCUS PNEUMONIAE	GH20C
2	A, B	2-acetamido-2-deoxy-beta-D-galactopyranose	non-polymer		221.2	2	Chemie (NGA)
3		water	water		18.0	622	Chemie (HOH)

Sequence modifications

A, B: 1 - 626 (UniProt: C1CH75)

PDB	External Database	Details
Met -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Leu 9	Pro 9	conflict
Val 158	Met 158	conflict
Gln 223	Glu 223	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	148361.1
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	148803.5

*Water molecules are not included.