5ABO
CRYSTAL STRUCTURE ANALYSIS OF FUNGAL VERSATILE PEROXIDASE FROM PLEUROTUS ERYNGII. MUTANT VPi-br. MUTATED RESIDUES T2K, D69S, T70D, S86E, A131K, D146T, Q202L, Q219K, H232E, Q239R, L288R, S301K, A308R, A309K AND A314R.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | VERSATILE PEROXIDASE VPL2 | polymer | 331 | 35028.5 | 1 | UniProt (O94753) Pfam (PF00141) Pfam (PF11895) | PLEUROTUS ERYNGII (BOLETUS OF THE STEPPES) | VERSATILE LIQUID PHASE PEROXIDASE 2, VERSATILE PEROXIDASE |
| 2 | B, C (A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 3 | D (A) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 1 | Chemie (HEM) | |||
| 4 | E (A) | water | water | 18.0 | 449 | Chemie (HOH) |
Sequence modifications
A: 3 - 331 (UniProt: O94753)
| PDB | External Database | Details |
|---|---|---|
| Ala 1 | - | expression tag |
| Lys 2 | - | expression tag |
| Ser 69 | Asp 99 | engineered mutation |
| Asp 70 | Thr 100 | engineered mutation |
| Glu 86 | Ser 116 | engineered mutation |
| Lys 131 | Ala 161 | engineered mutation |
| Thr 146 | Asp 176 | engineered mutation |
| Leu 202 | Gln 232 | engineered mutation |
| Lys 219 | Gln 249 | engineered mutation |
| Glu 232 | His 262 | engineered mutation |
| Arg 239 | Gln 269 | engineered mutation |
| Arg 288 | Leu 318 | engineered mutation |
| Lys 301 | Ser 331 | engineered mutation |
| Arg 308 | Ala 338 | engineered mutation |
| Lys 309 | Ala 339 | engineered mutation |
| Arg 314 | Ala 344 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 35028.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 696.6 | |
| All* | Total formula weight | 35725.1 |
