5A78

Crystal structure of the homing endonuclease I-CvuI in complex with I- CreI target (C1221) in the presence of 2 mM Mg revealing DNA not cleaved

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA ENDONUCLEASE I-CVUI	polymer	172	19292.5	2	UniProt (P56347) Pfam (PF00961)	CHLORELLA VULGARIS	23S RRNA INTRON PROTEIN
2	C, D (C, D)	24MER DNA, 5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP *CP*GP*AP*CP*GP*TP*TP*TP*TP*GP*A)-3'	polymer	24	7368.8	2		SYNTHETIC CONSTRUCT
3	E, F (B, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	G, H, I (A, B, C)	water	water		18.0	22	Chemie (HOH)

Sequence modifications

A, B: 3 - 162 (UniProt: P56347)

PDB	External Database	Details
Ala 2	-	expression tag
Ser 163	-	expression tag
Ala 164	-	expression tag
Ala 165	-	expression tag
Leu 166	-	expression tag
Glu 167	-	expression tag
His 168	-	expression tag
His 169	-	expression tag
His 170	-	expression tag
His 171	-	expression tag
His 172	-	expression tag
His 173	-	expression tag
Gln 54	Arg 53	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	53322.6
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	53371.2

*Water molecules are not included.