5A2C

Crystal Structure of Anoxybacillus Alpha-amylase Provides Insights into a New Glycosyl Hydrolase Subclass

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	ALPHA-AMYLASE	polymer	497	58164.4	1	UniProt (I1VWH9) Pfam (PF00128) Pfam (PF22026)	ANOXYBACILLUS SP.
2	B (B)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	C, D, E, F (A)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
4	G (A)	water	water		18.0	518	Chemie (HOH)

Sequence modifications

A: 24 - 478 (UniProt: I1VWH9)

PDB	External Database	Details
Met -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Ser 0	-	expression tag
Ser 1	-	expression tag
Gly 2	-	expression tag
Leu 3	-	expression tag
Val 4	-	expression tag
Pro 5	-	expression tag
Arg 6	-	expression tag
Gly 7	-	expression tag
Ser 8	-	expression tag
His 9	-	expression tag
Met 10	-	expression tag
Ala 11	-	expression tag
Ser 12	-	expression tag
Met 13	-	expression tag
Thr 14	-	expression tag
Gly 15	-	expression tag
Gly 16	-	expression tag
Gln 17	-	expression tag
Gln 18	-	expression tag
Met 19	-	expression tag
Gly 20	-	expression tag
Arg 21	-	expression tag
Gly 22	-	expression tag
Ser 23	-	expression tag
Leu 479	-	expression tag
Glu 480	-	expression tag
His 481	-	expression tag
His 482	-	expression tag
His 483	-	expression tag
His 484	-	expression tag
His 485	-	expression tag
His 486	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	58164.4
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	4
	Total formula weight	160.3
All*	Total formula weight	58667.0

*Water molecules are not included.