5A0S

Apo-structure of metalloprotease Zmp1 variant E143A from Clostridium difficile

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ZINC METALLOPROTEASE ZMP1	polymer	198	21925.6	2	UniProt (Q183R7) Pfam (PF07737)	CLOSTRIDIUM DIFFICILE
2	C, D (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
3	E, F (A, B)	water	water		18.0	107	Chemie (HOH)

Sequence modifications

A, B: 27 - 220 (UniProt: Q183R7)

PDB	External Database	Details
Gly 23	-	expression tag
Ser 24	-	expression tag
His 25	-	expression tag
Met 26	-	expression tag
Ala 143	Glu 143	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	43851.3
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	43982.1

*Water molecules are not included.