4ZWU
Crystal structure of organophosphate anhydrolase/prolidase mutant Y212F, V342L, I215Y
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | ORGANOPHOSPHATE ANHYDROLASE/PROLIDASE | polymer | 440 | 50453.6 | 2 | UniProt (Q44238) Pfam (PF21216) Pfam (PF00557) | Alteromonas sp. | X-Pro dipeptidase,DFPase,Imidodipeptidase,Organophosphorus acid anhydrolase 2,OPAA-2,Paraoxon hydrolase,Phosphotriesterase,Proline dipeptidase,Prolidase |
| 2 | C, D, F, I, J... (A, B) | MANGANESE (II) ION | non-polymer | 54.9 | 6 | Chemie (MN) | |||
| 3 | E, L (A, B) | BARIUM ION | non-polymer | 137.3 | 2 | Chemie (BA) | |||
| 4 | G (B) | GLYCOLIC ACID | non-polymer | 76.1 | 1 | Chemie (GOA) | |||
| 5 | H (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | M, N (A, B) | water | water | 18.0 | 470 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 437 (UniProt: Q44238)
| PDB | External Database | Details |
|---|---|---|
| Thr 210 | Asn 210 | variant |
| Pro 211 | Ala 211 | variant |
| Phe 212 | Tyr 212 | engineered mutation |
| Tyr 215 | Ile 215 | variant |
| Asn 314 | Asp 314 | engineered mutation |
| Leu 342 | Val 342 | engineered mutation |
| Glu 438 | - | expression tag |
| Leu 439 | - | expression tag |
| Asp 440 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 100907.2 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 772.4 | |
| All* | Total formula weight | 101679.7 |
