4ZWN

Crystal Structure of a Soluble Variant of the Monoglyceride Lipase from Saccharomyces Cerevisiae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Monoglyceride lipase	polymer	334	38040.1	4	UniProt (P28321) Pfam (PF12146)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	MGL,Monoacylglycerol hydrolase,MGH,Monoacylglycerol lipase,MAGL,Serine hydrolase YJU3
2	E (A)	NITRATE ION	non-polymer		62.0	1	Chemie (NO3)
3	F, G (B, C)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	H (D)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	I, J, K, L (A, B, C, D)	water	water		18.0	408	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 313 (UniProt: P28321)

PDB	External Database	Details
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Asp -14	-	expression tag
Ile -13	-	expression tag
Pro -12	-	expression tag
Thr -11	-	expression tag
Thr -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Ser 175	Leu 175	engineered mutation
Arg 264	Gln 264	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	152160.3
Non-Polymers*	Number of molecules	4
	Total formula weight	277.1
All*	Total formula weight	152437.4

*Water molecules are not included.