4ZTZ

Structural basis for processivity and antiviral drug toxicity in human mitochondrial DNA replicase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase subunit gamma-1	polymer	1222	138037.7	1	UniProt (P54098) Pfam (PF18136)	Homo sapiens (Human)	Mitochondrial DNA polymerase catalytic subunit,PolG-alpha
2	B, C (B, C)	DNA polymerase subunit gamma-2, mitochondrial	polymer	472	53655.2	2	UniProt (Q9UHN1) Pfam (PF03129)	Homo sapiens (Human)	DNA polymerase gamma accessory 55 kDa subunit,p55,Mitochondrial DNA polymerase accessory subunit,MtPolB,PolG-beta
3	D (T)	DNA (25-MER)	polymer	27	8235.3	1		synthetic construct
4	E (P)	DNA (5'-D(P*AP*AP*GP*AP*CP*GP*AP*GP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*GP*TP*AP*C)-3')	polymer	24	7421.8	1		synthetic construct
5	F, G (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	H (A)	2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE	non-polymer		467.2	1	Chemie (DCP)

Sequence modifications

A: 30 - 1239 (UniProt: P54098)

PDB	External Database	Details
Met 29	-	expression tag
Ala 198	Asp 198	conflict
Ala 200	Glu 200	conflict
Ala 1240	-	expression tag
Ala 1241	-	expression tag
Ala 1242	-	expression tag
Leu 1243	-	expression tag
Glu 1244	-	expression tag
His 1245	-	expression tag
His 1246	-	expression tag
His 1247	-	expression tag
His 1248	-	expression tag
His 1249	-	expression tag
His 1250	-	expression tag

B, C: 26 - 485 (UniProt: Q9UHN1)

PDB	External Database	Details
Met 25	-	expression tag
Ala 486	-	expression tag
Ala 487	-	expression tag
Ala 488	-	expression tag
Leu 489	-	expression tag
Glu 490	-	expression tag
His 491	-	expression tag
His 492	-	expression tag
His 493	-	expression tag
His 494	-	expression tag
His 495	-	expression tag
His 496	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	261005.1
Non-Polymers*	Number of molecules	3
	Total formula weight	515.8
All*	Total formula weight	261520.9

*Water molecules are not included.