4ZS4

Crystal Structure of the Inactive Alpha-kinase Domain of Myosin-II Heavy Chain Kinase A (D756A) Complexed with ATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Myosin heavy chain kinase A	polymer	307	34482.6	2	UniProt (P42527) Pfam (PF02816) In PDB	Dictyostelium discoideum (Slime mold)	MHCK-A
2	A, B	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
3	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	A, B	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
5		water	water		18.0	322	Chemie (HOH)

Sequence modifications

A, B: 552 - 841 (UniProt: P42527)

PDB	External Database	Details
Met 535	-	initiating methionine
Gly 536	-	expression tag
Gly 537	-	expression tag
His 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag
Gly 544	-	expression tag
Glu 545	-	expression tag
Asn 546	-	expression tag
Leu 547	-	expression tag
Tyr 548	-	expression tag
Phe 549	-	expression tag
Gln 550	-	expression tag
Gly 551	-	expression tag
Ala 756	Asp 756	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	68965.1
Non-Polymers*	Number of molecules	6
	Total formula weight	1335.1
All*	Total formula weight	70300.3

*Water molecules are not included.