4ZJ3

Crystal structure of cephalexin bound acyl-enzyme intermediate of Val216AcrF mutant TEM1 beta-lactamase from Escherichia coli: E166N and V216AcrF mutant.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-lactamase TEM	polymer	297	33328.1	1	UniProt (P62593) Pfam (PF00144) Pfam (PF13354)	Escherichia coli	IRT-4,Penicillinase,TEM-1,TEM-16/CAZ-7,TEM-2,TEM-24/CAZ-6,TEM-3,TEM-4,TEM-5,TEM-6,TEM-8/CAZ-2
2	B (A)	water	water		18.0	307	Chemie (HOH)

Sequence modifications

A: 3 - 290 (UniProt: P62593)

PDB	External Database	Details
4ov 70	Ser 68	engineered mutation
Ile 84	Val 82	see remark 999
Asn 166	Glu 164	engineered mutation
Val 184	Ala 182	see remark 999
4ou 216	Val 214	engineered mutation
Ala 291	-	expression tag
Ala 292	-	expression tag
Ala 293	-	expression tag
Leu 294	-	expression tag
Glu 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	33328.1
All*	Total formula weight	33328.1

*Water molecules are not included.