4ZJ2

Crystal Structure of p-acrylamido-phenylalanine modified TEM1 beta-lactamase from Escherichia coli :E166N mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-lactamase TEM	polymer	297	33211.0	1	UniProt (P62593) Pfam (PF00144) Pfam (PF13354)	Escherichia coli	IRT-4,Penicillinase,TEM-1,TEM-16/CAZ-7,TEM-2,TEM-24/CAZ-6,TEM-3,TEM-4,TEM-5,TEM-6,TEM-8/CAZ-2
2	B (A)	water	water		18.0	213	Chemie (HOH)

Sequence modifications

A: 3 - 290 (UniProt: P62593)

PDB	External Database	Details
Ile 84	Val 82	see remark 999
Asn 166	Glu 164	engineered mutation
Val 184	Ala 182	see remark 999
Ala 291	-	expression tag
Ala 292	-	expression tag
Ala 293	-	expression tag
Leu 294	-	expression tag
Glu 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	33211.0
All*	Total formula weight	33211.0

*Water molecules are not included.