4ZJ1

Crystal Structure of p-acrylamido-phenylalanine modified TEM1 beta-lactamase from Escherichia coli : V216AcrF mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Beta-lactamase TEM	polymer	297	32995.7	1	UniProt (P62593) Pfam (PF00144) Pfam (PF13354) In PDB	Escherichia coli	IRT-4,Penicillinase,TEM-1,TEM-16/CAZ-7,TEM-2,TEM-24/CAZ-6,TEM-3,TEM-4,TEM-5,TEM-6,TEM-8/CAZ-2
2	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
3	A	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
4	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5		water	water		18.0	468	Chemie (HOH)

Sequence modifications

A: 3 - 290 (UniProt: P62593)

PDB	External Database	Details
Ile 84	Val 82	see remark 999
Val 184	Ala 182	see remark 999
4ou 216	Val 214	engineered mutation
Ala 291	-	expression tag
Ala 292	-	expression tag
Ala 293	-	expression tag
Leu 294	-	expression tag
Glu 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	32995.7
Non-Polymers*	Number of molecules	3
	Total formula weight	393.5
All*	Total formula weight	33389.2

*Water molecules are not included.