4ZJ1
Crystal Structure of p-acrylamido-phenylalanine modified TEM1 beta-lactamase from Escherichia coli : V216AcrF mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Beta-lactamase TEM | polymer | 297 | 32995.7 | 1 | UniProt (P62593) Pfam (PF00144) Pfam (PF13354) | Escherichia coli | IRT-4,Penicillinase,TEM-1,TEM-16/CAZ-7,TEM-2,TEM-24/CAZ-6,TEM-3,TEM-4,TEM-5,TEM-6,TEM-8/CAZ-2 |
| 2 | B (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 3 | C (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 4 | D (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 5 | E (A) | water | water | 18.0 | 468 | Chemie (HOH) |
Sequence modifications
A: 3 - 290 (UniProt: P62593)
| PDB | External Database | Details |
|---|---|---|
| Ile 84 | Val 82 | see remark 999 |
| Val 184 | Ala 182 | see remark 999 |
| 4ou 216 | Val 214 | engineered mutation |
| Ala 291 | - | expression tag |
| Ala 292 | - | expression tag |
| Ala 293 | - | expression tag |
| Leu 294 | - | expression tag |
| Glu 295 | - | expression tag |
| His 296 | - | expression tag |
| His 297 | - | expression tag |
| His 298 | - | expression tag |
| His 299 | - | expression tag |
| His 300 | - | expression tag |
| His 301 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 32995.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 393.5 | |
| All* | Total formula weight | 33389.2 |
