4ZIR

Crystal structure of EcfAA' heterodimer bound to AMPPNP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Energy-coupling factor transporter ATP-binding protein EcfA2	polymer	267	30333.1	1	UniProt (Q9WY65) Pfam (PF00005) In PDB	Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)	ECF transporter A component EcfA2, TmEcfA
2	B	Energy-coupling factor transporter ATP-binding protein EcfA1	polymer	268	29859.9	1	UniProt (Q9X1Z1) Pfam (PF00005) In PDB	Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)	ECF transporter A component EcfA1, TmEcfA'
3	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	A, B	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
5	A, B	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
6		water	water		18.0	20	Chemie (HOH)

Sequence modifications

A: 2 - 266 (UniProt: Q9WY65)

PDB	External Database	Details
Met 0	-	expression tag
Gly 1	-	expression tag

B: 2 - 259 (UniProt: Q9X1Z1)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Arg 2	Lys 2	engineered mutation
Glu 4	Thr 4	engineered mutation
Ala 53	Glu 53	engineered mutation
Ala 55	Glu 55	engineered mutation
Ala 125	Glu 125	engineered mutation
Ala 126	Lys 126	engineered mutation
Ala 127	Glu 127	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60193.0
Non-Polymers*	Number of molecules	7
	Total formula weight	1167.4
All*	Total formula weight	61360.4

*Water molecules are not included.