4ZFT

Catalytic domain of Sst2 F403W mutant bound to ubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	AMSH-like protease sst2	polymer	197	22020.8	2	UniProt (Q9P371) Pfam (PF01398)	Schizosaccharomyces pombe (Fission yeast)	Suppressor of ste12 deletion protein 2
2	B, D (B, D)	Polyubiquitin-B	polymer	81	8988.3	2	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (Human)
3	E, F, J, K (A, C)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
4	G, H, I, L, M (A, B, C, D)	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
5	N, O, P, Q (A, B, C, D)	water	water		18.0	79	Chemie (HOH)

Sequence modifications

A, C: 245 - 435 (UniProt: Q9P371)

PDB	External Database	Details
Gly 239	-	expression tag
Pro 240	-	expression tag
Leu 241	-	expression tag
Gly 242	-	expression tag
Ser 243	-	expression tag
Met 244	-	expression tag
Trp 403	Phe 403	engineered mutation

B, D: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	62018.1
Non-Polymers*	Number of molecules	9
	Total formula weight	572.0
All*	Total formula weight	62590.1

*Water molecules are not included.