4ZF8
Cytochrome P450 pentamutant from BM3 with bound Metyrapone
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Bifunctional P-450/NADPH-P450 reductase | polymer | 468 | 53501.0 | 1 | UniProt (P14779) Pfam (PF00067) | Bacillus megaterium | Cytochrome P450(BM-3),Cytochrome P450BM-3 |
| 2 | B (A) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 1 | Chemie (HEM) | |||
| 3 | C (A) | METYRAPONE | non-polymer | 226.3 | 1 | Chemie (MYT) | |||
| 4 | D, E, F, G (A) | NICKEL (II) ION | non-polymer | 58.7 | 4 | Chemie (NI) | |||
| 5 | H (A) | water | water | 18.0 | 83 | Chemie (HOH) |
Sequence modifications
A: 0 - 460 (UniProt: P14779)
| PDB | External Database | Details |
|---|---|---|
| Leu 47 | Arg 48 | engineered mutation |
| Ile 81 | Phe 82 | engineered mutation |
| Val 87 | Phe 88 | engineered mutation |
| Gln 188 | Leu 189 | engineered mutation |
| Val 267 | Glu 268 | engineered mutation |
| Thr 461 | - | expression tag |
| His 462 | - | expression tag |
| His 463 | - | expression tag |
| His 464 | - | expression tag |
| His 465 | - | expression tag |
| His 466 | - | expression tag |
| His 467 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 53501.0 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1077.5 | |
| All* | Total formula weight | 54578.5 |
