4ZBJ

UBN1 peptide bound to H3.3/H4/Asf1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Histone chaperone ASF1	polymer	175	19664.0	1	UniProt (P32447) Pfam (PF04729)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Anti-silencing function protein 1,yASF1
2	B (B)	Histone H3	polymer	77	8812.3	1	UniProt (Q10453) Pfam (PF00125) UniProt (by SIFTS) (Q6PI79)	Xenopus laevis (African clawed frog)
3	C (C)	Histone H4	polymer	84	9540.3	1	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
4	D (D)	Ubinuclein-1	polymer	28	3144.2	1	UniProt (Q9NPG3) Pfam (PF08729)	Homo sapiens	HIRA-binding protein,Protein VT4,Ubiquitously expressed nuclear protein
5	E, F, G, I, J... (A, B, C)	CHLORIDE ION	non-polymer		35.5	8	Chemie (CL)
6	H (A)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
7	N, O, P, Q (A, B, C, D)	water	water		18.0	158	Chemie (HOH)

Sequence modifications

A: 2 - 169 (UniProt: P32447)

PDB	External Database	Details
Pro -5	-	expression tag
Leu -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Pro -1	-	expression tag
Asn 0	-	expression tag
Ser 1	-	expression tag

B: 61 - 135 (UniProt: Q10453)

PDB	External Database	Details
Met 59	-	initiating methionine
Ala 60	-	expression tag
Ala 102	Gly 103	engineered mutation

C: 20 - 102 (UniProt: P62799)

PDB	External Database	Details
Met 19	-	initiating methionine

D: 122 - 148 (UniProt: Q9NPG3)

PDB	External Database	Details
Ace 121	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	41160.8
Non-Polymers*	Number of molecules	9
	Total formula weight	401.8
All*	Total formula weight	41562.6

*Water molecules are not included.