4Z5Q

Crystal structure of the LnmZ cytochrome P450 hydroxylase from the leinamycin biosynthetic pathway of Streptomyces atroolivaceus S-140 at 1.8 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cytochrome P450 hydroxylase	polymer	423	47187.3	1	UniProt (Q8GGM6) Pfam (PF00067) In PDB	Streptomyces atroolivaceus
2	A	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	1	Chemie (HEM)
3	A	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
4	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	A	3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL	non-polymer		326.4	1	Chemie (P33)
6	A	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
7		water	water		18.0	154	Chemie (HOH)

Sequence modifications

A: 1 - 400 (UniProt: Q8GGM6)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Gln -11	-	expression tag
Asp -10	-	expression tag
Pro -9	-	expression tag
Gly -8	-	expression tag
Asp -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	47187.3
Non-Polymers*	Number of molecules	8
	Total formula weight	1325.0
All*	Total formula weight	48512.3

*Water molecules are not included.