4Z2T

Crystal Structure of 2-hydroxybiphenyl 3-monooxygenase W225Y from Pseudomonas azelaica

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	2-hydroxybiphenyl-3-monooxygenase	polymer	592	64642.2	2	UniProt (O06647) Pfam (PF01494) Pfam (PF21274)	Pseudomonas nitroreducens HBP1
2	C, D (A, B)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
3	E, F (A, B)	water	water		18.0	455	Chemie (HOH)

Sequence modifications

A, B: 2 - 586 (UniProt: O06647)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Tyr 225	Trp 225	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	129284.4
Non-Polymers*	Number of molecules	2
	Total formula weight	1571.1
All*	Total formula weight	130855.5

*Water molecules are not included.