4Z1Z
Crystal Structure of Meganuclease I-SmaMI Bound to Uncleaveable DNA with a TTCT Central Four
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Meganuclease I-SmaMI | polymer | 303 | 34283.8 | 2 | UniProt (F7WD42) Pfam (PF00961) | Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell) | |
| 2 | C, E (D, F) | DNA (28-MER) | polymer | 28 | 8412.4 | 2 | synthetic construct | ||
| 3 | D, F (C, E) | DNA (28-MER) | polymer | 28 | 8799.7 | 2 | synthetic construct | ||
| 4 | G, I (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 5 | H, J, K, L, M... (A, D, C, E) | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
| 6 | O, P, Q, R (A, B, C, F) | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
A, B: 6 - 301 (UniProt: F7WD42)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Ser 1 | - | expression tag |
| Lys 2 | - | expression tag |
| Gly 3 | - | expression tag |
| Glu 4 | - | expression tag |
| Asn 5 | - | expression tag |
| Asn 165 | Leu 278 | conflict |
| Gln 267 | Met 380 | conflict |
| Leu 302 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 102991.9 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 632.7 | |
| All* | Total formula weight | 103624.6 |
