4Z1Z
Crystal Structure of Meganuclease I-SmaMI Bound to Uncleaveable DNA with a TTCT Central Four
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Meganuclease I-SmaMI | polymer | 303 | 34283.8 | 2 | UniProt (F7WD42) Pfam (PF00961) In PDB | Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell) | |
2 | D, F | DNA (28-MER) | polymer | 28 | 8412.4 | 2 | synthetic construct | ||
3 | C, E | DNA (28-MER) | polymer | 28 | 8799.7 | 2 | synthetic construct | ||
4 | A, B | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
5 | A, D, C, E | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
6 | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
A, B: 6 - 301 (UniProt: F7WD42)
PDB | External Database | Details |
---|---|---|
Met 0 | - | initiating methionine |
Ser 1 | - | expression tag |
Lys 2 | - | expression tag |
Gly 3 | - | expression tag |
Glu 4 | - | expression tag |
Asn 5 | - | expression tag |
Asn 165 | Leu 278 | conflict |
Gln 267 | Met 380 | conflict |
Leu 302 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 102991.9 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 632.7 | |
All* | Total formula weight | 103624.6 |