4Z1X
Crystal structure of LAGLIDADG homing endonuclease I-GzeII in complex with DNA target
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | C, E | DNA (27-MER) | polymer | 27 | 8445.5 | 2 | synthetic construct | ||
2 | D, F | DNA (27-MER) | polymer | 27 | 8147.3 | 2 | synthetic construct | ||
3 | A, B | LAGLIDADG endonuclease | polymer | 299 | 34646.6 | 2 | UniProt (A5J036) Pfam (PF00961) In PDB | Fusarium graminearum PH-1 (Wheat head blight fungus) | I-GzeII |
4 | A, B | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
5 | water | water | 18.0 | 129 | Chemie (HOH) |
Sequence modifications
A, B: 7 - 300 (UniProt: A5J036)
PDB | External Database | Details |
---|---|---|
Asp 2 | - | expression tag |
Leu 3 | - | expression tag |
Ser 4 | - | expression tag |
Thr 5 | - | expression tag |
Ser 6 | - | expression tag |
Lys 48 | Leu 96 | engineered mutation |
Asn 56 | Ile 104 | engineered mutation |
Lys 106 | Leu 154 | engineered mutation |
Ser 154 | Val 202 | engineered mutation |
Asn 155 | Ile 203 | engineered mutation |
Asn 163 | Val 211 | engineered mutation |
Lys 265 | Leu 313 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 102478.7 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 160.3 | |
All* | Total formula weight | 102639.0 |